Figure 4.

PI3K inhibitor abrogates t-BHQ-mediated transcriptional events on the ferritin H ARE in Jurkat cells. Jurkat cells (1–2 × 10⁶) were pretreated with 0–50 μM LY294002 for 1 h, followed by treatment with or without 50 μM t-BHQ for 3 h. ChIP assays using antibodies against Nrf2, Bach1, acetyl histone H3-K9, -K14, -K18, p300, or CBP were performed along with rabbit IgG as a negative control. A representative autoradiograph and quantified ChIP DNA bands (-fold increases in y-axis) are shown from three independent experiments.