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EGFR-containing MVBs from SCAMP3-depleted cells have normal morphology. HeLa cells were transfected with control (A, B, E, F, and G) or SCAMP3 siRNA (C, D, H, I, and J), labeled with EGFR mAb 13A9 conjugated to gold, stimulated with 100 ng/ml EGF for 30 (A–D) or 60 min (E–J), and then processed for immunoelectron microscopy. Arrowheads indicate buds/tubules extending from the MVB. N, nucleus; LE, late endosome; bar, 0.25 μm.
