Skip to main content
. 2009 Mar 15;20(6):1661–1670. doi: 10.1091/mbc.E08-05-0525

Figure 8.

Figure 8.

Coilin on metaphase chromosomes. (A) Metaphase of an ovarian follicle cell, from a fly expressing GFP-labeled polo kinase. Stained with antibodies against coilin (red) and GFP (green). DNA is stained with DAPI (blue). Note that coilin is limited to a single row of dots along the center of the metaphase plate, suggesting that coilin lies between the centromeres of sister chromatids. In contrast, polo kinase occurs in two rows of dots, presumably the sister kinetochores oriented to opposite poles of the spindle. The poles themselves also contain polo kinase (arrows). An arrowhead indicates the plane of the metaphase plate. (B–E) Metaphase from a larval neuroblast stained with antibodies against coilin (red) and CID (green). CID is Drosophila CENP-A and defines the centromeres of the chromosomes. The arrowhead indicates the metaphase plate. Note that coilin is limited to the centromere regions on the metaphase plate and that CID dots lie on either side of the coilin (toward the poles of the spindle), again suggesting that coilin lies between the sister chromatids.