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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Nat Methods. 2008 Sep 15;5(11):947–949. doi: 10.1038/NMETH.1258

Figure 1.

Figure 1

Reactivation of EYFP-MreB fusions in the same live C. crescentus cell. (a–l) Fluorescence images of single EYFP-MreB molecules overlaid on a reversed-contrast white-light image of the cell being examined. Initial ‘single-molecule concentration’ image showing two isolated single molecules (a). A nonemissive cell after exposure to 514-nm excitation for a few seconds (b). A short 407-nm reactivation pulse was administered after frames b, d, f, h and j in all of which no molecules are in the emissive state. Reactivated single molecules are observed in subsequent frames (c,e,g,i,k). Scale bar, 1 µm.