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a, representative binding isotherm, here for Rac1-plexin-B1 binding, obtained from isothermal calorimetric titration experiments in phosphate buffer, pH 7.0, with 4 mm MgCl2 and 1 mm tris(2-carboxyethyl) phosphine (TCEP) at 25 °C. Top, raw data; bottom, peak integrated and concentration-normalized enthalpy change. b, isotherm for plexin-B1 monomer-dimer conversion under the same conditions as above. Top, raw data; bottom, peak integrated enthalpy change.
