TABLE 2. Molecular weights determined by gel filtration on a Superdex-75 column.
Only a single peak was observed in gel filtration elution profiles for plexin RBD complexes with Rac1, Rnd1, or RhoD GTPases and for mixtures (not shown). Rnd1 and Rnd1-RBD run at a larger Mr possibly due to protein shape (long unstructured termini).
| Proteins | ExpectedMr | ExperimentalMr |
|---|---|---|
| RBD W1830F | 13.4 | 13.0 |
| RBD dimer | 26.8 | 25.9 |
| Rac1 | 22.1 | 23.5 |
| Rac1 + plexin | 35.5 | 34.0 |
| Rnd1 | 24.6 | 32.6 |
| Rnd1 + plexin | 38.1 | 45.0 |
| RhoD | 21.4 | 18.3 |
| RhoD + plexin | 35.0 | 33.5 |