Table 3.

Wgef Is Regulated Like Ngef and Tim^a

			RhoA
Wgef			kobs (×10−3s−1)	fold stimulation
-			0.07	1.00
Δ285			0.11	1.53
Δ302			4.59	65.7
Δ285+S394A			0.43	6.19
Δ302+S394A			2.72	39.0
Δ285+Y295E			3.54	50.7
			RhoA
Wgef	Src	ATP	kobs (×10−3 s−1)	fold stimulation
−	−	−	0.04	1.00
+	−	−	0.09	2.06
+	−	+	0.11	2.52
+	+	−	0.13	3.12
+	+	+	0.31	7.36
−	+	+	0.05	1.14
			RhoA
Wgef	EphA4	ATP	kobs (×10−3 s−1)	fold stimulation
+	+	−	0.05	1.11
+	+	+	0.19	4.47
−	+	+	0.08	1.86

^aAn analysis of the representative exchange data presented in Figure 5A, is shown. k_obs and fold stimulation were calculated as described in Table 1.