Table 4.
Crystallographic statistics. Numbers in parentheses represent the highest resolution bin of width ∼ 0.06 Å.
| Complex I | Complex II | Complex III | |
|---|---|---|---|
| Space group | P213 | P213 | P213 |
| Unit cell length a = b = c (Å) | 137.1 | 136.9 | 137.2 |
| Resolution range (Å) | 40–2.1 | 40.0–2.25 | 40.0–2.30 |
| No. observed/unique reflections | 262 443/49 871 | 304 071/40 884 | 285 651/38 442 |
| Wilson B (Å2) | 32.8 | 37.8 | 39.4 |
| Completeness (%) | 99.4 (95.7) | 100.0 (100.0) | 100.0 (100.0) |
| Multiplicity/Rmerge (%) | 5.3 (4.1)/6.4 (45.2) | 7.4 (7.6)/13.7 (49.1) | 7.4 (7.4)/11.6 (50.4) |
| <I/σ(I)> | 17.1 (3.1) | 12.1 (2.9) | 14.2 (3.3) |
| Rwork/Rfree (%) | 20.8 (28.3)/25.2 (33.8) | 20.2 (24.8)/22.9 (29.6) | 19.7 (24.3)/24.5 (32.4) |
| r.m.s.d. from ideal bond lengths (Å)/angles (°) | 0.008/1.186 | 0.008/1.168 | 0.008/1.096 |
| Average B values (Å2) | |||
| Overall/main chain/ side chain/waters/ ligands | 30.9/30.4/31.4/38.8 (2 CDP 30.7; 2 ADP 43.8; 4 SO42− 53.9; 2 Cl− 23.1) | 30.9/30.3/31.4/36.0 (2 CDPME 30.9; 2 ADP 39.7; 3 SO42− 59.4; 3 Cl− 28.0) | 30.0/29.5/30.5/37.9 (2 CTN 41.1; 1 ligand 43.2; 8 SO42− 54.9; 2 Cl− 19.5) |
| Ramachandran plot analysis | |||
| Most favourable regions (%) | 94.3 | 94.9 | 94.0 |
| Additional allowed regions (%) | 5.3 | 4.6 | 5.5 |
| DPIa (Å) | 0.20 | 0.23 | 0.25 |
a Diffraction-Component Precision Index [43].