Table 1.

Proteins identified by 2D-DIGE as being differentially expressed along the crypt-villus axis in mouse small intestine. Values shown are the mean fold change computed from the 8 gels. Score (Probability-based mascot score) and Coverage (Sequence percentage coverage which peptides matched to) were calculated from mascot based on combined mass and mass/mass spectra.

Accession no.	Proteins Upregulated in the villus	Symbol	Biological Process	Fold change Villus/Crypt)	P Value	Score	# of peptides matched*	Coverage (%)
56206029	profilin 1a	PFN1	Ctoskeletal org. biogenesis	2	0.01	165	5,2	41
34980904	keratin 20	KRT20	Cell structure	1.84	0.00042	219	22,2	61
6678573	villin 1	VIL1	Cell structure	1.56	0.036	94	17,2	17
50881	Ezrin	VIL2	Cell structure	1.61	0.0081	572	51,5	53
1703127	actin, cytoplasmic	ACTB	Cell structure; Exo/Endocytosis;Cytokinesis;	1.71	0.027	359	22,5	44
7548322	aldolase 1, A isoformb	ALDOA	Glycolysis	1.65	0.0078	91	16,1	45
21707669	aldolase 2, B isoform	ALDOB	Glycolysis	2.25	0.0029	288	22,5	37
54673814	enolase 1, alpha non-neuron	ENO1	Glycolysis	1.61	0.019	541	33,8	53
28436836	triosephosphate isomerase 1	TPI1	Glycolysis;Other metabolism	1.56	0.02	103	10,1	33
31982229	ketohexokinase	KHK	Monosaccharide metabolism	1.8	0.019	80	11,2	33
56206644	malate dehydrogenase 1, NAD (soluble)	MDH1	Tricarboxylic acid pathway	1.88	0.0013	251	14,5	35
58037546	isocitrate dehydrogenase 1 (NADP+),soluble	IDH1	Tricarboxylic acid pathway	1.65	0.0049	438	35,7	54
19484047	malate dehydrogenase 2, NAD (mitochondrial)	MDH2	Tricarboxylic acid pathway	1.54	0.049	251	16,3	43
16741459	ATP synthase, H+ transp, mitochon.F0 complex, subunit d	ATP5H	ATP synthesis coupled proton transport	1.91	0.0033	118	10,2	54
2894517	fatty acid binding protein 1, livera	FABP1	Lipid and fatty acid transport	2	0.01	165	14,5	67
15215161	microsomal triglyceride transfer protein	MTTP	Lipid and fatty acid transport;Transport	1.54	0.0428	112	26,2	27
2492905	annexin A4	ANXA4	Lipid, fatty acid and steroid metabolism	1.56	0.0012	143	17,2	48
9845263	dopa/tyroine sulfotransferase	SULT1B1	Steroid hormone metabolism;Sulfur metab.	1.87	0.00063	115	11,3	47
2257955	cytochrome b-5	CYB5A	Steroid metab. Electr. transp.	1.54	0.049	120	6,1	80
397351	mCRBPII	RBP2	Steroid hormone metabolism	2.53	0.003	670	21,8	32
71059735	pyrophosphatase (inorganic) 1	PPA1	Polyphosphate catabolism	1.69	0.049	72	9,2	32
71059839	adenosine deaminase	ADA	Purine metabolism	6.43	0.0061	266	23,5	42
19526926	unnamed protein	C11orf54	unclassified	1.52	0.02	141	19,2	48
19484063	arginase type II	ARG2	Amino acid catabolism	1.63	0.0051	104	12,6	34
52789310	archain 1	ARCN1	Constitutive exocytosis	1.99	0.0045	118	22,3	26
Accession no.	Proteins downregulated in the villus	Symbol	Biological process	Fold change (Villus/Crypt)	P Value	Score	# of peptides matched*	Coverage (%)
53237015	peptidylprolyl isomerase A	Ppia	Protein folding;Nuclear transp;Immunity/defense	−1.61	0.03	105	8,2	43
76779273	heat shock protein 1 (chaperonin)	Hspd1	Protein folding/complex assembly	−1.93	0.019	359	24,6	39
38303969	heat shock 70kD protein 5 (glucose-regulated protein)	HSPA5	Protein folding/complex assembly; Stress resp.	−1.6	0.047	449	38,10	43
435839	mortalin	HSPA9	Protein folding/complex assembly; Stress resp.	−1.56	0.00044	674	32,8	61
123681	heat shock protein 1, beta	HSP90AB1	Protein folding; Stress resp.	−1.55	0.015	182	24,4	41
12860388	ribosome binding protein 1	Rrbp1	Protein localization; Cell structure and motility	−1.86	0.038	82	19,0	20
8393866	ornithine aminotransferase	OAT	Amino acid metab. Vision	−2.24	0.00081	691	35,10	50
20987331	eukaryotic translation initiation factor 3, subunit 2b	EIF3S2	Protein biosynthesis	−1.93	0.00028	197	17,2	46
9790219	destrin	DSTN	Cell structure	−1.52	0.0013	61	4,2	15
13542680	tubulin, beta 2c	TUBB2C	Protein traffic;C'some seg; Cell structure/motility	−1.56	0.025	287	32,5	40
20381452	Lgals2 protein	LGALS2	Cell adhesion; immune/defense; apoptosis	−1.5	0.016	384	15,6	69
37747847	heterogeneous nuclear ribonucleoprotein A2/B1	HNRPA2B1	mRNA splicing	−1.62	0.033	121	8,3	44
73918911	carbamoyl-phosphate synthetase 1	CPS1	Arginin, pyrimidine biosynthesis, urea cycle	−1.68	0.0057	476	58,7	48
31542333	hypoxia up-regulated 1	HYOU1	Stress response	−1.57	0.016	489	55,7	43
51770420	unknown protein	LOC433141	Biological process unclassified	−1.59	0.029	118	10,3	30
31560560	laminin receptor 1	LAMR1	Biological process unclassified	−1.68	0.0014	126	10,2	30
15426464	anterior gradient 2 (Xenopus laevis)	AGR2	Miscellaneous	−1.74	0.00042	128	9,4	42
Accession no.	Contradictory findings	Symbol	Biological process	Fold change (Villus/Crypt)	P Value	Score	# of peptides matched*	Coverage (%)
13624315	keratin complex 2, basic, gene 8	KRT8	Cell structure, Ectoderm development	2.11/−2.72	0.0085/0.028	343/344	43,8/42,5	52/59
6680606	keratin complex 1, acidic, gene 19	KRT19	Cell structure	2.82/−1.54	0.006/0.021	148/133	24,4/15,1	47/39
15488625	fatty acid binding protein 2, intestinal	FABP2	Lipid and fatty acid transport	2.41/−2.54	0.013/0.013	393/149	15,5/10,2	79/52
42542422	heat shock protein 8	Hspa8	Protein folding/complex assembly; Stress resp.	1.56/−1.57	0.028/0.021	508/258	40,7/22,5	47/43

Numbers shown in the “ # of peptides matched column” represent the number of peptides matched with either MS or MS/MS and with MS/MS, respectively.

^a,bindicates multiple proteins present in one spot."