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A, electron micrograph of an OHC hair bundle from a cochlear culture labelled with rabbit antibody PB221 to the ectodomain of TRPML3 followed by 10 nm gold-conjugated goat anti-rabbit. The gold particles are observed in the ankle-link region (arrows) at the base of the hair bundle. B, detail of ankle-link region shown above in A. Scale bars = 200 nm. C, labelling is not seen in the control with non-immune rabbit IgG.
