Figure 2. Effects of carboxy-terminal truncations on channel activity and surface expression.

A, schematic depictions of channel constructs used and confocal images of cells expressing WT hClC-2 or R751X hClC-2 channels fused to either mYFP (upper) or HA epitope tagged after immunodetection with Alexa 488-conjugated antibody (lower). B, bar plot of the relative amount of channels expressed at the plasma membrane (average of 4 separate experiments) and schematic depictions of the construct used. C, neutralization of the protopore gate (E205Q) recovers the function of truncated R751X hClC-2. Pulse protocol and representative current recordings of tsA201 cells expressing E205Q/R751X hClC-2 channels, before and after the application of 10 mm Cd²⁺. D, current–voltage relationships for R751X hClC-2 (n = 15), R751X hClC-2 in external pH 6 (n = 8), and E205Q/R751X hClC-2 before (n = 6) and after (n = 4) applying 10 mm Cd²⁺.