Table 1.

qRT-PCR primer sequences

Gene name	Forward sequence	Reverse sequence
IGF-1Ea	5′-GACATGCCCAAGACCCAGAAGGA-3′	5′-CGGTGGCATGTCACTCTTCACTC-3′
IGF-1Eb	5′-GCCCCCATCTACCAACAAGAACAC-3′	5′-CAGACTTGCTTCTGTCCCCTCCTTC-3′
MGF	5′-GCCCCCATCTACCAACAAGAACAC-3′	5′-CGGTGGCATGTCACTCTTCACTC-3′
MyoD	5′-GGTCCCTCGCGCCCAAAAGAT-3′	5′-CAGTTCTCCCGCCTCTCCTAC-3′
Myf5	5′-ATGGACGTGATGGATGGCTG-3′	5′-GCGGCACAAACTCGTCCCCAA-3′
MRF4	5′-CCCCTTCAGCTACAGACCCAA-3′	5′-CCCCCTGGAATGATCGGAAAC-3′
Myogenin	5′-CAGTGCACTGGAGTTCAGCG-3′	5′-TTCATCTGGGAAGGCCACAGA-3′
GAPDH	5′-CCTCCTGCACCACCAACTGCTT-3′	5′-GAGGGGCCATCCACAGTCTTCT-3′

IGF-1, insulin-like growth factor-1; MGF, mechano growth factor (IGF-1Ec); MyoD, myogenic determination factor; Myf5, myogenic factor-5; MRF4, myogenic regulatory factor-4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.