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. 2008 Oct 9;586(Pt 23):5757–5769. doi: 10.1113/jphysiol.2008.160887

Figure 4. CD34 and CXCR4 expression in the peripheral blood cells and in hearts.

Figure 4

A, representative pictures showing the CD34 (red), CXCR4 (green), and nucleus (DAPI, blue) staining in peripheral blood obtained from one control and one HPC rat. The colocalization signal is indicated by arrowheads in the merged picture. Magnification at 100×. The inset pictures in the DAPI image show the colocalization of CD34, CXCR4, and nucleus at high magnification. Horizontal bar = 100 μm. B, the upper panel shows representative blots for the mRNA expression of CXCR4 (224 bases), CD34 (492 bases), and GAPDH (535 bases) (n = 2 for each group). Lower bar graph shows the ratio of the DUs for CXCR4 and CD34 compared with GAPDH, respectively (n = 5 for each). M, 100 bp DNA ladder. NTC, ‘no template control’ indicates that the primers were replaced by diethylpyrocarbonate water during PCR. *P < 0.05 compared to the control group. †P < 0.05 compared to the IR group.