Figure 4. Effect of inhibition of PI3 kinase on [Ca²⁺]_o- and calcimimetic-regulated branching morphogenesis.

A, effect of 1.05 mm${\text{Ca}}_{\text{o}}^{2+}$ (upper panels), 1.05 mm${\text{Ca}}_{\text{o}}^{2+}$ plus 30 nm R-568 (middle panels) and 1.7 mm${\text{Ca}}_{\text{o}}^{2+}$ (lower panels) in the absence (0.05% DMSO vehicle control – 1st and 2nd panels) or presence (3rd and 4th panels) of 12.5 μm of the PI3K inhibitor LY294002 on branching. Bar = 750 μm. B, mean percentage change in branching at 24 and 48 h. Rescue by LY294002 of the inhibition of branching which was evoked by either R-568 or 1.7 mm${\text{Ca}}_{\text{o}}^{2+}$ is indicated in time-matched samples by the asterisks (**P < 0.01).