Table 4.
Skeletal muscle morphology and capillarization in the plantaris in untrained and exercise-trained muscle of WT and AMPK DN mice
| Untrained | Exercise trained | P value | |||||
|---|---|---|---|---|---|---|---|
| WT (n = 8) | AMPK DN (n = 9) | WT (n = 8) | AMPK DN (n = 10) | Interaction | Transgene | Exercise | |
| Capillary contacts | 4.46 ± 0.14 | 4.10 ± 0.28 | 5.22 ± 0.21 | 5.10 ± 0.24 | 0.60 | 0.30 | <0.01 |
| Capillary-to-fibre ratio | 1.58 ± 0.06 | 1.41 ± 0.11 | 1.94 ± 0.09 | 1.88 ± 0.11 | 0.59 | 0.26 | <0.01 |
| Capillary density (capillaries mm−2) | 1425 ± 104 | 1063 ± 73 | 1230 ± 106 | 1218 ± 90 | 0.08 | 0.05 | 0.83 |
| CFPE (capillaries (1000 mm)−1) | 11.17 ± 0.49 | 9.13 ± 0.62 | 11.50 ± 0.56 | 11.18 ± 0.30 | 0.09 | 0.02 | 0.02 |
| Fibre area (μm2) | 1187 ± 52 | 1446 ± 88 | 1783 ± 142 * | 1575 ± 128 | 0.05 | — | — |
| Fibre perimeter (μm) | 140 ± 3 | 153 ± 4 | 172 ± 8* | 161 ± 7 | 0.05 | — | — |
| Fibre-type composition (%) | |||||||
| Type I | 21.1 ± 2.5 | 21.5 ± 3.7 | 22.1 ± 1.1 | 25.1 ± 1.6 | 0.60 | 0.51 | 0.37 |
| Type IIa | 43.4 ± 2.7 | 33.3 ± 2.6* | 55.1 ± 4.1*# | 59.0 ± 2.6*# | 0.03 | — | — |
| Type IIb | 35.5 ± 3.4 | 45.2 ± 5.0 | 22.8 ± 4.3*# | 15.9 ± 1.6*# | 0.04 | — | — |
Mean ± s.e.m. CFPE, capillary-to-fibre perimeter exchange index.
*
Significantly different from untrained WT.
#
Significantly different from untrained AMPK DN.