TABLE 1.

Strains and plasmids used

Strain or plasmid	Genotype/relevant characteristicsa	Source or reference
Strains
E. coli K-12 BW25113	lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBA-DAH33 ΔrhaBADLD78	3
E. coli K-12 BW25113 sdiA	K-12 BW25113 ΔsdiA Ω Kmr	3
E. coli K-12 BW25113 tnaA	K-12 BW25113 ΔtnaA Ω Kmr	3
E. coli K-12 BW25113 mtr	K-12 BW25113 Δmtr Ω Kmr	3
E. coli K-12 BW25113 csgB	K-12 BW25113 ΔcsgB Ω Kmr	3
E. coli K-12 BW25113 csgE	K-12 BW25113 ΔcsgE Ω Kmr	3
E. coli K-12 BW25113 csgF	K-12 BW25113 ΔcsgF Ω Kmr	3
E. coli K-12 BW25113 adrA	K-12 BW25113 ΔadrA Ω Kmr	3
E. coli K-12 BW25113 tnaA sdiA	K-12 BW25113 ΔtnaA ΔsdiA Ω Kmr	This study
E. coli K-12 BW25113 mtr sdiA	K-12 BW25113 Δmtr ΔsdiA Ω Kmr	This study
Plasmids
pCA24N	Empty vector; Cmr	28
pCA24N-sdiA	pCA24N pT5-lac::sdiA; expresses SdiA derived from E. coli	28
pCA24N-adrA	pCA24N pT5-lac::adrA; expresses AdrA derived from E. coli	28
pCP20	Apr and Cmr plasmid with temperature-sensitive replication and thermal induction of FLP synthesis	10

^aKm^r, Cm^r, and Ap^r stand for kanamycin, chloramphenicol, and ampicillin resistance, respectively.