TABLE 2.

Primers used for epPCR, DNA sequencing, RT-PCR, and confirmation of the double mutants

Purpose	Primer	Sequence
epPCR of SdiA	SdiA front	5′-AGGCGTATCACGAGGCCCTTTC-3′
	SdiA reverse	5′-CAGTCACGATGAATTCCCCTAG-3′
DNA sequencing	Seq primer 1	5′-CACCGATCGCCCTTCCCAACAGTTGC-3′
	Seq primer 2	5′-GCAGTTACTGGTGCGCGAAAG-3′
RT-PCR for tnaA and housekeeping	TnaA front	5′-TGAAGAAGTTGGTCCGAATAACGTG-3′
gene rrsG	TnaA reverse	5′-CTTTGTATTCTGCTTCACGCTGCTT-3′
	RrsG front	5′-TATTGCACAATGGGCGCAAG-3′
	RrsG reverse	5′-ACTTAACAAACCGCCTGCGT-3′
Confirmation of the sdiA mutation	SdiA up	5′-AATGCGATGGCTTGCAAAAGTAATT-3′
	SdiA down	5′-AGCAAATTAAACAAGCCTACCGTCA-3′
Confirmation of the tnaA mutation	TnaA up	5′-CTGGCGAATTAATCGGTATAGCAGA-3′
	TnaA down	5′-GATCAGTCATGATGCCACCTTTAGA-3′
Confirmation of the mtr mutation	Mtr up	5′-GTACTCGTGTACTGGTACAGTGCAA-3′
	Mtr down	5′-TCCTACATAGACCTGATAAGCGAAG-3′