TABLE 2.
Oligonucleotide primers used in this studyf
| Primer | Sequenceg |
|---|---|
| SOE-P1-CspA-A | GGAATTCCGGAAAACAAACAAGTGACGCTa |
| SOE-P1-CspA-B | TGCTTGAGGTCCGCGACCTTGTTCCATGTTCATGTTCCT |
| SOE-P1-CspA-C | AGGAACATGGAACAAGGTCGCGGACCTCAAGCAGCTe |
| SOE-P1-CspA-D | ACGCGTCGACCACCATCACCGATTATCGGAATAb |
| SOE-P2-CspB-A | TCCCCCGGGGCGTCCGGCAAGTGTGAATTGGTTAGTGAGCGAGc |
| SOE-P2-CspB-B | TGCTTGTGGGCCACGAACTGTACCTGTTTGCATATTTCACAA |
| SOE-P2-CspB-C | AACAGGTACAGTTCGTGGCCCACAAGCAGAAAAAGe |
| SOE-P2-CspB-D | TCCCCCGGG CGCACTGGAATACAAGCTGGGACTATGCCCGCTTTc |
| SOE-P3-CspD-A | TCCCCCGGGCCACCATTTTTGTTACAGAGGAAGGAc |
| SOE-P3-CspD-B | CAATGCAAAAT GGAAAGTACGCGGCGCTCAAG CAG |
| SOE-P3-CspD-C | CTGCTTGAGCGCCGCGTACTTTCCCATTTTGCATTGAAATAAATCCe |
| SOE-P3-CspD-D | TCCCCCGGGCGCACTGGAATACAAGCTGGGACTATGCCCGCTTTc |
| CspA-comp-P1 | AAAACTGCAG CTGATTTAATCGCACTTAGAGAAAATTAATCAd |
| CspA-comp-P2 | TCCCCCGGG TTACGCTTTTTGAACGTTAGCTGCTTc |
| CspA-fw | AACATGGAACAAGGTACAG* |
| CspA-rv | GTTGGCCTTCTTCAACG* |
| CspB-fw | CAAACAGGTACAGTTAAATGGTTTA* |
| CspB-rv | ACGATTTCAAATTCAACGCTTTGA* |
| CspD-fw | TACGGTTTTATCGAATCAGAC* |
| CspD-rv | ACGTTAGCTGCTTGAG* |
| 16S rRNA-fw | CTTCCGCAATGGACGAAAGT* |
| 16S rRNA-rv | CTCATCGTTTACGGCGTG* |
The EcoRI restriction site incorporated in this primer to facilitate cloning is underlined.
The SalI restriction site incorporated in this primer to facilitate cloning is underlined.
The SmaI restriction site incorporated in this primer to facilitate cloning is underlined.
The PstI restriction site incorporated in this primer to facilitate cloning is underlined.
The regions complementary to SOE-P1-CspA-B, SOE-P1-CspB-B, and SOE-P1-CspD-B primers are in italics.
Oligonucleotides were synthesized at Microsynth AG (Balgach, Switzerland). Primers were designed using the LightCycler probe and primer design software (Roche Molecular Diagnostics GmBH, Penzburg, Germany).
*, quantitative real-time RT-PCR primer.