TABLE 1.
Primers used in RT-PCRs
| Primer | Sequence | Applicationa |
|---|---|---|
| A-F | 5′GAATTCCCTGCCGGCGCAACTGACC | Sense lsdA (1395) |
| A-R | 5′GTTCTGCGCACCGTTCTGATACAGC | Antisense lsdA (1977) |
| B-F | 5′GAGGAGATCTTTTCCGGAAGCCTGG | Sense lsdB (3204) |
| B-R | 5′GTCCAGCTTCAGCGGAACCAGTGTG | Antisense lsdB (3632) |
| AB-F | 5′CCTGGGCGGCTATGGCGATATTCCG | Sense lsdA/lsdB junction (2667) |
| AB-R | 5′CGTTCGGATCGTTCATCCAGTACGC | Antisense lsdA/lsdB junction (3054) |
a
Each number in parentheses refers to the position of the primer's 5′ end in the SRT4 lsdAB operon sequence as available in the DDBJ/EMBL/GenBank databases under accession number L41732.