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Purification of ST0053-ADH from recombinant E. coli cellsa
| Step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Crude extract | 167.0 | 24.7 | 0.15 | 100 | 1 |
| Heat treatment | 17.6 | 23.2 | 1.33 | 95 | 8.9 |
| Butyl Sepharoseb | 6.8 | 9.6 | 1.41 | 39 | 9.4 |
Wet weight of cells, about 2.55 g.
The values were determined after concentration with ammonium sulfate.
