TABLE 2.

Comparison of primary structures of the products from 10 putative ADH genes annotated in the genome of S. tokodaii and medium-chain ADHs so far found and characterized

Enzymea	% Amino acid sequence homology with:
	ST0053	ST0038	ST0075	ST0480	ST0569	ST2056	ST2252	ST2577	ST2605	STS015	SSADH	RC3ADH	APADH	YADH	HLADH
STADHs
ST0053	100	37	26	29	37	28	29	55	33	37	55	55	40	26	23
ST0038		100	24	19	28	30	26	36	29	24	35	34	48	24	23
ST0075			100	31	30	27	25	27	24	18	27	26	26	23	21
ST0480				100	22	29	26	29	26	29	29	28	26	26	20
ST0569					100	31	26	33	23	31	32	32	38	22	24
ST2056						100	25	32	28	22	31	30	29	27	38
ST2252							100	28	54	22	25	26	27	22	17
ST2577								100	30	33	89	89	40	28	22
ST2605									100	12	28	29	27	26	17
STS015										100	37	35	57	29	29
mADHs
SSADH											100	94	39	29	24
RC3ADH												100	40	30	23
APADH													100	27	20
YADH														100	23
HLADH															100

^aSTADHs, ADH genes annotated in the genome of S. tokodaii; mADHs, medium-chain ADHs; SSADH, S. solfataricus DSM 1617 (1JVB_A); RC3ADH, Sulfolobus sp. strain RC3; APADH, A. pernix K1 (APE_2239.1); YADH, Saccharomyces cerevisiae (P00330); HLADH, horse liver (1P1R_A).