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. 2009 Jan 9;75(6):1758–1763. doi: 10.1128/AEM.01392-08

TABLE 3.

Kinetic constants of major substrates

Substrate Vmax (μmol · mg−1 · min−1) Km (mM) Vmax/Km (mg−1 · min−1 · ml)
Oxidative reaction
    Ethanol 1.34 9.08 0.148
    1-Propanol 1.15 1.21 0.950
    1-Butanol 1.41 0.129 10.9
    1-Pentanol 1.18 0.00754 156
    1-Hexanol 1.32 0.0594 22.2
    2-Butanol 3.76 632 0.00595
    2-Pentanol 0.482 17.1 0.0282
    2-Hexanol 0.860 13.3 0.0647
    Cyclohexanol 0.511 5.72 0.0893
    Benzyl alcohol 2.09 0.0265 78.9
    1,3-Propanediol 1.16 16.8 0.0690
    NAD (0.5 mM benzyl alcohol) 2.05 0.0415 49.4
Reductive reaction
    Acetaldehyde 16.1 29.7 0.542
    1-Propanal 20.0 7.61 2.63
    1-Butanal 40.5 2.29 17.7
    1-Pentanal 24.2 0.613 39.5
    1-Hexanal 13.5 1.82 7.42
    Benzaldehyde 59.9 0.766 78.2
    NADH (3 mM benzaldehyde) 39.1 2.10 18.6