TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Sequence
Deletions in siiE
SiiE-Del-Ig21-Tet-For	5′-CGGCACGAAAGAGGTGCTGACGGCCACCAAAGACGCGACCTTAAGACCCACTTTCACA-3′
SiiE-Del-Ig21-Tet-Rev	5′-CATCTGCCCATGTGCCGGTCGGTGTCACGCTCCAGTTGCCCTAAGCACTTGTCTCCTG-3′
SiiE-Del6541-12492-For	5′-CGGCACGAAAGAGGTGCTGCTGACGGCCACCAAAGACGCGACCGGCGAATGGAGTTGGACGCCGCCATCAGTATTAGCGCCAG-3′
SiiE-Del6541-12492-Rev	5′-CTGGCGCTAATACTGATGGCGGCGTCCAACTCCATTCGCCGGTCGCGTCTTTGGTGGCCGTCAGCACCTCTTTCGTGCCG-3′
SiiE-Del6541-9540-For	5′-CGGCACGAAAGAGGTGCTGACGGCCACCAAAGACGCGACCGACGGCTGGCGCTATCGACCGGATTCTGCTTTGGCGGACG-3′
SiiE-Del6541-9540-Rev	5′-CGTCCGCCAAAGCAGAATCCGGTCGATAGCGCCAGCCGTCGGTCGCGTCTTTGGTGGCCGTCAGCACCTCTTTCGTGCCG-3′
SiiE-Del6541-8037-For	5′-CGGCACGAAAGAGGTGCTGACGGCCACCAAAGACGCGACCGGGATCTGGGATTACACCTGGCCGAAAGATGTGACCGACG-3′
SiiE-Del6541-8037-Rev	5′-CGTCGGTCACATCTTTCGGCCAGGTGTAATCCCAGATCCCGGTCGCGTCTTTGGTGGCCGTCAGCACCTCTTTCGTGCCG-3′
Site-directed mutagenesis of siiF
SiiF502-503-TetAR-For	5′-CATACCTGCGGGGCAACGTGTCGCGGTGGTAGGCGAATGCTTAAGACCCACTTTCACAtt-3′
SiiF502-503-TetAR-Rev	5′-GTAGCCAGATAGCATTCCCAGTAATGAGCTTTTTCCTGCTCCCTAAGCACTTGTCTCCTG-3′
SiiF-TetSwap-G500E-For	5′-GACATACCTGCGGGGCAACGTGTCGCGGTGGTAGaaGAATGCGGAGCCGGAAAAAGCTCATTACTGGGAATGCTATCTGGCTAC-3′
SiiF-TetSwap-G500E-Rev	5′-GTAGCCAGATAGCATTCCCAGTAATGAGCTTTTTCCGGCTCCGCATTCTTCTACCACCGCGACACGTTGCCCCGCAGGTATGTC-3′
SiiF-TetSwap-K506L-For	5′-CATACCTGCGGGGCAACGTGTCGCGGTGGTAGGCGAATGCGGAGCCGGATTAAGCTCATTACTGGGAATGCTATCTGGCTACCTTT-3′
SiiF-TetSwap-K506L-Rev	5′-AAAGGTAGCCAGATAGCATTCCCAGTAATGAGCTTAATCCGGCTCCGCATTCGCCTACCACCGCGACACGTTGCCCCGCAGGTATG-3′
SiiF-TetSwap-SacI-For	5′-CATACCTGCGGGGCAACGTGTCGCGGTGGTAGGCGAATGCGGAGCCGGAAAGAGCTCATTACTGGGAATGCTATCTGGCTACCT-3′
SiiF-TetSwap-SacI-Rev	5′-AGGTAGCCAGATAGCATTCCCAGTAATGAGCTCTTTCCGGCTCCGCATTCGCCTACCACCGCGACACGTTGCCCCGCAGGTATG-3′
Control primers
TetAR-Red-Check-For	5′-GATCAAGAGCATCAAGTCGC-3′
TetAR-Red-Check-Rev	5′-TCAGCAAGGTGCTTTACAGG-3′