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In vitro complementation of the catalase mutant. Bacterial H2O2 resistance was assessed by a disk diffusion inhibition assay and the zone of inhibition was measured in centimeters. A representative assay is shown for the wild type, TAM82 (ΔcpeB::Kmr), and TAM107 (ΔcpeB::Kmr NS1::Cmr cpeB+). The experiment was performed in triplicate, and the following P values were obtained: wild type versus TAM82, <0.01; wild type versus TAM107, >0.3; TAM82 versus TAM107, <0.04.
