TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| X. fastidiosa strains | ||
| Temecula1 | Wild-type Pierce's disease isolate, ATCC 700964 | 10 |
| Fetzer | Wild-type isolate | 13 |
| Stags Leap | Wild-type isolate | 13 |
| Traver | Wild-type isolate | 13 |
| UCLA | Wild-type isolate | 13 |
| TAM22 | Temecula1, NS1::Cmr | This study |
| TAM132 | Temecula1, NS1::Emr | This study |
| TAM105 | Temecula1, NS1::Gmr | This study |
| TAM91 | Temecula1, NS1::Kmr | This study |
| TAM82 | Temecula1, ΔcpeB::Kmr | This study |
| TAM107 | TAM82, NS1::CmrcpeB+ | This study |
| E. coli TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL endA1 nupG | Invitrogen |
| Plasmids | ||
| pBBR1MCS-5 | pBBR1 replicon, broad-host-range cloning vector, Gmr | 18 |
| pCR-Blunt II-TOPO | Blunt PCR cloning vector, Kmr | Invitrogen |
| pGEM-T | pMB1 replicon, TA cloning vector, Apr | Promega |
| pRL1342 | RSF1010 replicon, broad-host-range cloning vector, Cmr Emr | 42 |
| pXF004 | RSF1010::Kmr, broad-host-range vector | 12 |
| pXF005 | RSF1010Δ201::Kmr, broad-host-range vector | 12 |
| pAX1 | pGEM-T with intergenic region between PD0702 and PD0703, Apr | This study |
| pAX1Cm | pAX1 with Cm resistance cassette and multiple-cloning site | This study |
| pAX1Em | pAX1 with Er resistance cassette and multiple-cloning site | This study |
| pAX1Gm | pAX1 with Gm resistance cassette and multiple-cloning site | This study |
| pAX1Km | pAX1 with Km resistance cassette and multiple-cloning site | This study |
| pAM75 | pGEM-T with cpeB-flanking regions (ΔcpeB), Apr | This study |
| pAM92 | pAM75 ΔcpeB::Kmr | This study |
| pAM102 | pCR-Blunt II-TOPO with wild-type cpeB, Kmr | This study |
| pAM110 | pAX1Cm::cpeB+ | This study |