TABLE 1.
Bacterial strains and plasmidsa
| Plasmid or strain | Characteristics | Source |
|---|---|---|
| Strains | ||
| NCTC11168 | HS:2 | NCTC; 17 |
| NCTC11168s | Streptomycin-resistant derivative | This study |
| NCTC11168Δ38-44 | Streptomycin sensitive, Catr; genes between Cj1138c and Cj1144c deleted | This study |
| NCTC11168Δ32-52 | Streptomycin resistant, Kanr; genes between Cj1132c and Cj1152c (gmhB) deleted | This study |
| Plasmids | ||
| pUC19 | Ampicillin resistant | New England BioLabs |
| pGLM8 | pUC19 with a fragment containing BamHI-digested Catr and XbaI-digested rpsLWT | This study |
| pGLM9 | pUC19 with a fragment of XbaI/XhoI-digested Cj1138c, XhoI/KpnI-digested Cj1144c, and XhoI-digested CatrrpsLWT cassette from pGLM8 | This study |
| pGLM13 | pUC19 with a fragment of XbaI/XhoI-digested Cj1132c, XhoI/KpnI-digested Cj1152c, and XhoI-digested CatrrpsLWT cassette from pGLM8 | This study |
| pGLM14 | pGLM13 digested with XhoI and then blunted and ligated with EcoRV-digested Kanr | This study |
a
NCTC, National Collection of Type Cultures; WT, wild type.