TABLE 2.
Primers
| Primer name | Nucleotide sequence (5′ to 3′)a | Product or annealing siteb |
|---|---|---|
| Strep(XbaI)F | GCTCTAGAGCTCAAAGAAAGGAATTATTGTGCCTACC | rpsL |
| Strep(XbaI)R | GCTCTAGAGCTCTAACGGATTTGTCTGTATGAATG | rpsL |
| wlaPF(XbaI) | GCTCTAGAGCTTAGATTCTGATGATTATTGGGAATTAAAC | Cj1138c |
| wlaPR(XhoI) | CCGCTCGAGCGGTACAAATCGCACTAATTCTAAACTG | Cj1138c |
| wlaSCF(XhoI) | CCGCTCGAGCGGGTTGTCCATAAACTCCCATTTG | Cj1144c |
| wlaSCR(KpnI) | GGGGTACCCCGGTATGGGTAGATCTTGATATG | Cj1144c |
| CATrpsLF(XhoI) | CCGCTCGAGCGGTTTTCATACCAATTTTTAAGCTCTGCTCGGCGGTGTTCCTTTC | CatrrpsLWT cassette |
| CATrpsLR(XhoI) | CCGCTCGAGCGGTTGTTGTTCTAACAATAATTTTAGCTCTAACGGATTTGTCTGTATGAATG | CatrrpsLWT cassette |
| CATrpsLF1(XhoI) | CCGCTCGAGCGGTTTTAATAACAATCTTGTTGTTCTGCTCGGCGGTGTTCCTTTCCAAG | CatrrpsLWT cassette |
| CATrpsLR1(XhoI) | CCGCTCGAGCGGAAAAGTATGGTTAAAAATTGGAAGCTCTAACGGATTTGTCTGTATGAATG | CatrrpsLWT cassette |
| CATrpsLR2(XhoI) | CCGCTCGAGCGGTTTTAATAACAATCTTGTTGTTAGCTCTAACGGATTTGTCTGTATGAATG | CatrrpsLWT cassette |
| CATrpsLF2(XhoI) | CCGCTCGAGCGGAAAAGTATGGTTAAAAATTGGAAGCTCTAACGGATTTGTCTGTATGAATG | CatrrpsLWT cassette |
| wlaAF(XbaI) | GCTCTAGAGCCATTACCTCATCGCCATGCACATC | Cj1132c |
| wlaAR(XhoI) | CCGCTCGAGCGGGTGCCAGATGTTGAGCTTATCC | Cj1132c |
| Cj1152F(XhoI) | CCGCTCGAGCGGAAGTGCCAATATCAGCATTTAATCC | Cj1152c |
| wlaTR(KpnI) | GGGGTACCCCGGTTTAGATAGAGACGGTGTGATTAATATAG | Cj1152c |
a
The additional 5′ oligonucleotide sequence is underlined.
b
WT, wild type.