TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype, gene locus present, or description | Source |
|---|---|---|
| E. coli strains | ||
| HK100 | Expression strain derived from GeneHogs (Invitrogen) | JCSG |
| TK2420 | F−thi rha lacZ nagA Δ(kdpFAB)5 Δ(trkA-mscL′) trkD1 | W. Epstein (6) |
| Plasmids | ||
| pTM1088A/B | TM1088A, TM1088B | JCSG |
| pTM1057 | TM1057 | JCSG |
| pTM1088A | TM1088A | This study |
| pTM1088A/B-TM1089 | TM1088A, TM1088B, TM1089 | This study |
| pTM1088B-TM1089 | TM1088B, TM1089 | This study |
| pTM1089 | TM1089 | This study |
| pTM1088A/B G14A G16A G19A TM1089 | TM1088A G14AG16AG19A, TM1088B, TM1089 | This study |
| pTM1088A G14A G16A G19A | TM1088A G14AG16AG19A | This study |
| pTM1088B RCK | Amino acids 1-136 of TM1088B | This study |
| pMT2765/6 | MT2756, MT2766 | This study |
| pSpeedET no insert | No insert added | JCSG |
| pSpeedET | Expression vector, arabinose inducible, kanamycin resistance | JCSG |