TABLE 2.
Complementation of K+ transport activity of TK2420 on modified NaK solid medium with added KCl
| Construct | Growtha with KCl addition (mM)
|
||
|---|---|---|---|
| 0 | 2 | 4 | |
| pTM1088A/B | − | (−) | + |
| pTM1057 | − | + | + |
| pTM1088A/B-TM1089 | + | + | + |
| pTM1088B-TM1089 | (−) | + | + |
| pTM1089 | − | (−) | + |
| pTM1088A/B G14A G16A G19A TM1089 | + | ND | ND |
| pSpeedET no insert | − | (−) | + |
+, good growth; (−), barely visible growth; −, no growth. ND, not determined.