Growth curves of S. meliloti 1021 derivative strains in MM (A), MM supplemented with 0.5 M NaCl (B), or MM supplemented with 0.7 M sucrose (C). Only the growth curves of mutants significantly different from the wild type are shown. 1021 (wild type; black squares), 10tAG (Trk−; black triangles), 10tAK2 (Trk−, Kup2−; white diamonds), and 10tAKdp (Trk− Kdp−; asterisks) are analyzed. Data are representative of at least two replicate experiments. OD600, optical density at 600 nm.