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. 2009 Jan 16;191(7):2296–2306. doi: 10.1128/JB.01430-08

TABLE 5.

Effect of Dns on natural transformation frequencies of C. jejuni

Straina Natural transformation frequencyb
C356 −
C356dns::cat (2.4 ± 0.6) × 10−06c
CNET002 (2.5 ± 0.2) × 10−05
CNET002(pWM1007Pr1492) (2.7 ± 1.4) × 10−05
CNET002(pWM1007Pr1492dns) (2.1 ± 1.8) × 10−08c
a

To determine the natural transformation frequency of C. jejuni strains C356 and C356dns::cat, homologous pUOA13 plasmid DNA (kanamycin resistance marker) was used; for strains CNET002, CNET002(pWM1007Pr1492), and CNET002(pWM1007Pr1492dns) homologous chromosomal DNA was used (chloramphenicol resistance marker).

b

The natural transformation frequency was determined by dividing the number of transformants ml−1 by the total number of CFU per ml−1. The values are the averages of three experiments. −, below the detection limit (5.6 × 10−10 ± 5.0 × 10−10), which is based on a minimum of one colony per plate, adapted to the volumes used in the procedure.

c

P < 0.05 (Student's t test) for a comparison with the corresponding parental strain.