TABLE 2.

Primers used in this study

Gene-primer name and purpose	Sequence (5′→3′)	PCR product size (bp)
Gene inactivation
rirA-BT1201	GGCTTACGGCGTATCTGAGC	188
rirA-BT1202	CGTCCTCAAAGCACTCAGCC
Complementation
rirA-BT1701	GCGGTCAACAAGGCTGTTCG	593
rirA-BT1205	CGATCAGGCTGCCGGAAGTG
RT-PCR
16S rRNA-BT1421	GAATCTACCCATCTCTGCGG	280
16S rRNA-BT1422	AAGGCCTTCATCACTCACGC
fhuA-BT1346	GGTGACGAAGGGTATTGGCG	202
fhuA-BT1096	CGCGACATAACCTTTCACCG
fbpA-BT2728	CTGCGGATGTGCTGCTGACG	190
fbpA-BT2729	GTCACCCGCTCCTTCGAAGC
irp6A-BT2734	CACCGTCAAGGATGTGACCG	232
irp6A-BT2735	CTTCATGCCGCCGAAAGTCG
sodBI-BT534	CAGCACTACAACCACGTC	198
sodBI-BT535	TTCAAGCTTGCCGTTCTT
sodBII-BT641	GCGCAGTCCGGCATATTCA	200
sodBII-BT640	ACCAGCCACGCCCAGCCCG