TABLE 2.
Primers used to detect Salmonella prophage attachment sites via qPCRa
| Oligonucleotide | Sequence (5′ to 3′) | Product |
|---|---|---|
| Fels-1 attL up | GCAGATTGAGTACACGCAGC | Fels-1 attL |
| Fels-1 attL down | TTCTGCGAAAGGTACTATCTGCG | |
| Fels-1 attR up | ATTGGCCGGAACAACCAG | Fels-1 attR |
| Fels-1 attR down-2 | GTGTAGCTTCACGCTGGC | |
| Gifsy-1 attL up | TCCATCGAATCAAGTACCTGAGC | Gifsy-1 attL |
| Gifsy-1 attL down | GTGGAATGTCCACCGCTG | |
| Gifsy-1 attR up | TTGCGGGGATCTTGAGAGG | Gifsy-1 attR |
| Gifsy-1 attR down | GTAGTGTAGAATGCGGCGTTTC | |
| Gifsy-2 attL up | CCGCTGGAGTATACCTTGTTTAGC | Gifsy-2 attL |
| Gifsy-2 attL down | GTGGGATGTCAGAGAAGAGCG | |
| Gifsy-2 attR up | GGTCTGTGAAGTTCGTTAAAGTTCG | Gifsy-2 attR |
| Gifsy-2 attR down | CAAATCGCGCTACGCAGAATG | |
| sulA up | CCTACCTTCGCTGCTTCAAC | sulA |
| sulA down | CAGTCTTCAGGTTTGCCATT | |
| rpe for | CCAGCTCACTGCGCATTTCA | rpe |
| rpe rev | CCGCATTGATGCGTCCGGTT |
a
The attB sites of any of the phages can be amplified using the appropriate combination of attL up and attR down primers specific for that phage (up signifies upstream and down signifies downstream). Similarly, the attP sites can be amplified by using the appropriate combination of attR up and attL down primers.