TABLE 3.
Differential expression of membrane-associated proteins in 35000HP::P2AB relative to that in 35000HP
| NCBI RefSeq accession no. | Protein | Function | COG assignment | No. of peptidesa | Fold change in expressionb |
|---|---|---|---|---|---|
| NP_872657.1 | FrdA | Fumarate reductase (flavoprotein subunit) | C | 2 | 4.2 |
| NP_872698.1 | NapB | Nitrate reductase (cytochrome c subunit) | C | 4 | 1.7 |
| NP_872776.1 | NudH | Dinucleoside polyphosphate hydrolase | LR | 2 | 2.2 |
| NP_872819.1 | ClpX | Periplasmic protease | O | 4 | ND |
| NP_872924.1 | RluB | Probable pseudouridylate synthase | J | 2 | 33.3 |
| NP_872976.1 | RnfC | Iron-sulfur binding NADH dehydrogenase | C | 5 | ND |
| NP_873015.1 | SuhB | Inositol-1-monophosphatase | G | 2 | 5.9 |
| NP_873097.1 | PurT | Phosphoribosylglycinamide formyltransferase | F | 4 | ND |
| NP_873171.1 | AccC | Acetyl coenzyme A carboxylase (biotin carboxylase subunit) | I | 3 | ND |
| NP_873182.1 | HD0646 | Ligand-gated channel (CirA superfamily) | S | 1 | ND |
| NP_873191.1 | RpsL | 30S ribosomal protein S12 | J | 4 | 2.0 |
| NP_873197.1 | SecB | Chaperone/export protein | U | 2 | ND |
| NP_873221.1 | RpoD | RNA polymerase general sigma factor | K | 3 | 2.6 |
| NP_873288.1 | RpmF | 50S ribosomal protein L32 | J | 2 | ND |
| NP_873292.1 | HD0776 | Protein of unknown function | S | 3 | ND |
| NP_873489.1 | PutP | Proline permease | ER | 2 | 6.7 |
| NP_873522.1 | ArgH | Argininosuccinate lyase | E | 2 | 3.9 |
| NP_873554.1 | RecB | Exodeoxyribonuclease V (beta subunit) | L | 2 | 25.0 |
| NP_873651.1 | HD1190 | Predicted OM protein (OmpH-like) | M | 2 | 2.0 |
| NP_873672.1 | HD1219 | Protein of unknown function | S | 3 | ND |
| NP_873728.1 | RarA | DNA recombination factor | L | 8 | ND |
| NP_873766.1 | Kgd | Alpha-ketoglutarate decarboxylase | C | 4 | ND |
| NP_873801.1 | HD1377 | Protein of unknown function | S | 3 | 6.3 |
| NP_873812.1 | MoaE | Molybdopterin converting factor (subunit 2) | H | 1 | ND |
| NP_873822.1 | HD1400 | Putative soluble lytic murein transglycosylase | M | 2 | 2.1 |
| NP_873873.1 | Pta | Phosphate acetyltransferase | CR | 3 | ND |
| NP_873912.1 | ManB | Probable phosphomannomutase | G | 4 | ND |
| NP_873974.1 | MukB | Chromatin remodeling | D | 2 | 2.1 |
| NP_873995.1 | Rnc | RNase III | K | 3 | ND |
| NP_874012.1 | AceF | Dihydrolipoamide acetyltransferase | C | 11 | ND |
| NP_874147.1 | GroEL | 60-kDa chaperonin | O | 12 | 3.3 |
| NP_874148.1 | GroES | Co-chaperonin with GroEL | O | 4 | 2.0 |
| NP_874162.1 | RpmI | 50S ribosomal protein L35 | J | 3 | 3.3 |
| NP_874219.1 | RpoB | DNA-directed RNA polymerase (beta subunit) | K | 2 | ND |
| NP_874225.1 | NusG | Transcription antitermination protein | K | 2 | 3.6 |
| NP_874227.1 | HD1887 | Putative oligopeptide transporter | S | 1 | ND |
| NP_873852.1 | OmpP2B | Porin | M | 1 | ND* |
| NP_873623.1 | LspA2 | Hemagglutinin-like macrophage phagocytic inhibitor | U | 30 | −2.0 |
| NP_874013.1 | AceE | Pyruvate dehydrogenase | C | 2 | −1.9 |
| NP_874019.1 | CafA | RNase G | J | 2 | −9.4 |
| NP_874039.1 | HD1654 | Protein of unknown function | S | 2 | −4.0 |
| NP_874075.1 | Imp | LPS/LOS export and organic solvent tolerance protein | M | 3 | −1.6 |
| NP_874111.1 | Uup | ABC-type transport protein | R | 2 | −1.5 |
| NP_874280.1 | RpsP | 30S ribosomal protein S16 | J | 3 | −1.5 |
| NP_874286.1 | RpmJ | 50S ribosomal protein L36 | J | 8 | −2.0 |
| NP_873133.1 | HD0591 | Putative two-component sensor kinase of LemA family | S | 3 | −1.5 |
| NP_873235.1 | FabG | 3-Oxoacyl-(acyl carrier protein) reductase | IQR | 1 | ND* |
| NP_873285.1 | DsrA | Serum resistance protein | UW | 1 | ND* |
| NP_873911.1 | LspA1 | Hemagglutinin-like macrophage phagocytic inhibitor | U | 10 | −2.0 |
| NP_874095.1 | NhaB | Na+/H+ antiporter protein | P | 1 | ND* |
| NP_874191.1 | Gpt | Xanthine-guanosine phosphoribosyltransferase | F | 1 | ND* |
| NP_874224.1 | RplK | 50S ribosomal protein L11 | J | 1 | ND* |
| NP_874256.1 | RpoZ | DNA-directed RNA polymerase omega subunit | K | 4 | −2.0 |
| NP_874282.1 | RpoA | DNA-directed RNA polymerase alpha subunit | K | 4 | −2.0 |
| NP_874285.1 | RpsM | 30S ribosomal protein S13 | J | 2 | −2.0 |
| NP_874312.1 | RpsJ | 30S ribosomal protein S10 | J | 5 | ND* |
a
Number of peptides used to identify the protein by LC-MS-MS analysis. The average differentially expressed protein was identified by 3.7 ± 0.6 peptides.
b
Several proteins were present in detectable quantities in one but not both samples, preventing quantitative analysis of protein expression. ND, not detected in 35000HP; ND*, not detected in 35000HP::P2AB.