TABLE 2.

Primers used for gene inactivation

Primer	Sequencea	Application
5′clpBArm1SphI	5′-GTTGAGGAAGCATGCCGTTCGGGC-3′	clpB deletion
3′clpBArm1BamHI	5′-CATTTTATCGGATCCAAAGTCACC-3′	clpB deletion
5′clpBArm2BamHI	5′-GGTGACTTTGGATCCGATAAAATG-3′	clpB deletion
3′clpBArm2SpeI	5′-CACCTGAATACTAGTCCAAAACGG-3′	clpB deletion
5′clpCArm1	5′-GGGATAAAATCATTCCTGGT-3′	clpC deletion
3′clpCArm1SphI	5′-TAATCGGTCATGCATGCTATCCTTTTCT-3′	clpC deletion
5′clpCArm2SphI	5′-GGACAGACTGCATGCATAGGTATTC-3′	clpC deletion
3′clpCArm2	5′-ACCCGTTTGGGCATCACG-3′	clpC deletion
5′clpEArm1	5′-GATCTGATAAGGCAGTAATAAA-3′	clpE deletion
3′clpEArm1BamHI	5′-GACAGAGCATGGATCCAATACCCCT-3′	clpE deletion
5′clpEArm2BamHI	5′-CTTCAGATTAGGATCCTCAGGTCTTTC-3′	clpE deletion
3′clpEArm2	5′-GAATGAAGCTGTCTAAG-3′	clpE deletion
clpLFwdSphI	5′-AGCTGGTGCGCATGCATCTTCATT-3′	clpL mutation
clpLRevSpeI	5′-GCCGATTGGACTAGTGCCTTCATC-3′	clpL mutation
5′clpPArm1	5′-GAAACGCCGATTACTAAGACTGTAC-3′	clpP deletion
3′clpPArm1BamHI	5′-ACTGTATTTCGGATCCAATTCAATCTTGG-3′	clpP deletion
5′clpPArm2BamHI	5′-CGCTTCTAGCGGATCCAAAGGAAAACG-3′	clpP deletion
3′clpPArm2	5′-CAAATTTAAGAGCACCAATC-3′	clpP deletion
5′clpXArm1	5′-GGCATGTACAGTGAAC-3′	clpX deletion
3′clpXArm1BamHI	5′-CAGCCATTTGGATCCCTAACTTCTAA-3′	clpX deletion
5′clpXArm2BamHI	5′-CTAAGAAAGCGGATCCAGGGACCGA-3′	clpX deletion
3′clpXArm2	5′-CTGACCTTCATTTTCAAA-3′	clpX deletion
5′spx1Arm1	5′-GTATTGTAAACGTACATG-3′	spxA deletion
3′spx1Arm1SphI	5′-GGTAACCATGCATGCACTACCCCTT-3′	spxA deletion
5′spx1Arm2SphI	5′-AGAAGATGACGCATGCGAATTACGC-3′	spxA deletion
3′spx1Arm2	5′-CAACTTCATTTTAGGAAT-3′	spxA deletion
5′spx2Arm1	5′-GTTTTATTCTTCTGTGCG-3′	spxB deletion
3′spx2Arm1EcoRI	5′-GTTTTAATCGAATTCTCCTTACAG-3′	spxB deletion
5′spx2Arm2HindIII	5′-GAATGTTCGAAGCTTTGAAAAGTC-3′	spxB deletion
3′spx2Arm2	5′-CTTTCTTTTTCTATAAGCAAAAAG-3′	spxB deletion

^aRestriction sites used to facilitate ligation are highlighted in bold.