TABLE 2.
Primer combinations and expected amplicon sizes of the three ICESde3396 regions targeted in the PCR screening of clinical GGS, GBS, and GAS isolatesa
| Primer | Nucleotide sequence (5′ to 3′) | Locationa | Expected size (kb)a | Source or reference |
|---|---|---|---|---|
| R1F | CTACTTTTTGTTGCCATTTGG | Amplification of the R1 region (orf1 to orf5) in ICESde3396 | 3.1 | This study |
| R1R | GAGTCAGTGTCCAAACTTTTCG | |||
| R2F | GTTTAAATCCCAGATAAGCTACG | Amplification of the R2 region (orf17 to orf23) | 4.2 | This study |
| R2R | ATCGATTATGATTTTGGTCAACG | |||
| R3F | TTCCTACTTGTACCAATACTGC | Amplification of the R3 region (orf55 to orf61) | 3.2 | This study |
| R3R | GAGTGACTTAGTTATGAAGGACG | |||
| Adapter1 | GATCCGCCTATAGTGAGTCGTATTAAC | Adapter PCR | 18, 43 | |
| Adapter2 | AGCTCGCCTATAGTGAGTCGTATTAAC |
a
ORFs from ICESde3396.