FIG. 3.

L. monocytogenes pathogenesis requires lipoate-dependent metabolism. Exponentially growing bacteria were mixed and injected intraperitoneally into 4- to 6-week-old female C57BL/6 mice. After 24 h, spleens and livers were harvested and plated onto LB agar with and without Erm. The CI was calculated by dividing the number of drug-sensitive (WT) CFUs by the number of drug-resistant (AlA2⁻ mutant) CFUs. The horizontal solid lines represent the median values, and the dotted upper line represents mice for which no AlA2⁻ CFUs were detected. The values from mice for which CFUs could not be detected were not used when CI values were calculated. Statistically significant differences between two groups were determined by Student's t test (P < 0.05, as indicated by the asterisks).