FIG. 3.
Northern blot analysis of sarA transcripts in Newman derivative ALC6094 and its isogenic sarA deletion mutant carrying pG164-MazF(His6)-pEPSA5-sarA. (A) The 1.2-, 0.8-, and 0.6-kb transcripts (long arrows) represent the native sarA transcripts from three distinct sarA promoters, which, as expected, were preserved upon MazFSa induction. (B) The ∼0.5-kb transcript (open arrow) represents the sarA transcript as driven from the modified pEPSA5 promoter in the plasmid pG164-MazFsa-pEPSA5-sarA. This transcript was preserved in the parental strain ALC6094 and the isogenic sarA mutant. Bottom, rRNA loading control. Total cellular RNAs were extracted at the indicated time points (minutes).