FIG. 5.

Decay of RNAs upon blocking of de novo RNA synthesis with rifampin. Rifampin (200 μg/ml) was added 30 min after the culture containing S. aureus ALC6094 harboring pG164-MazF(His₆) reached an OD₆₅₀ of 0.4. RNA was extracted from control samples at time zero (time when culture reached an OD₆₅₀ of 0.4) and at 15-min time points, shown in lanes 1 and 2, respectively, in panels A to D. Lanes 3 to 8 in panels A to D show the RNA extracted after the addition of rifampin for 0, 2, 4, 6, 10, and 15 min. (A to D) Transcripts detected with sarA, recA, hla, and sigB probes. 23S and 16S rRNA served as the internal loading controls.