TABLE 2.
Short-term 63Ni accumulation in H. pylori strains
| Genotype | Total 63Ni/cella
|
|||
|---|---|---|---|---|
| 26695
|
G27
|
|||
| Low Nib | High Nic | Low Nib | High Nic | |
| Wild type | 134.3 (1.9) | 8.52 (0.34) | 536.5 (40.3) | 6.45 (0.23) |
| nikR | 156.0 (7.5)* | 10.19 (0.79) | 867.5 (28.0)** | 6.99 (0.23)* |
| ureE | 92.2 (7.1)* | 6.86 (0.19)* | 196.8 (2.1)** | 5.26 (0.11)* |
| hypA | 128.1 (11.7) | 7.82 (0.45) | 191.2 (1.9)** | 8.71 (0.28)** |
| hypB | 124.6 (8.8)** | 5.46 (0.63)** | 213.6 (5.4)** | 5.18 (0.22)* |
| slyD | 134.0 (2.0) | 7.34 (0.36) | 564.0 (38.0) | 6.75 (0.17) |
| hypA slyD | 96.1 (1.8)** | 6.33 (0.41)** | 156.4 (5.5)** | 9.75 (0.08)** |
| ureAB | 120.7 (1.7)** | 7.68 (0.29) | 155.7 (5.1)** | 6.60 (0.35) |
| hydABC | 137.9 (5.1) | 8.09 (0.44) | ND | ND |
Values are the averages for three independent cultures (standard errors are in parentheses) for each strain and condition. Cells were incubated with 63NiCl2 as described in the text, harvested by centrifugation (16,000 × g, 1 min), washed with 900 μl of 50 mM HEPES (pH 7.0)-50 mM EDTA, and resuspended in 200 μl 10 mM acetic acid before being mixed with 1 ml scintilliation fluid. 63Ni levels were determined by scintillation counting using a preprogrammed 10-min acquisition window (0 to 1.31 MeV). Counts per minute were converted to atoms of 63Ni/CFU using a standard curve determined for cells grown under identical conditions. ND, not determined. * and **, P < 0.1 and P < 0.05 for mutant versus parent strain.
10 nM 63NiCl2.
50 nM 63NiCl2 plus 100 μM NiCl2. Values are in hundred thousands.