. 2009 Jan 30;191(7):2112–2121. doi: 10.1128/JB.01523-08

TABLE 3.

Intracellular metabolite concentrations and Gibbs energies of transhydrogenase reactions

Organism	Concn^a (μM) of:						NAD⁺/NADP⁺ ratio^a	Δ_rG′^b (kJ mol⁻¹) for:
Organism	Glucose-6P	6P-gluconate	NADP⁺	NAD⁺	NADPH	NADH	NAD⁺/NADP⁺ ratio^a	mTH	sTH
A. tumefaciens	522 ± 89	57 ± 6	203 ± 26	475 ± 20	138 ± 37	224 ± 26	2.34 ± 0.32	−8.01-−32.20	0.57-−2.21
S. meliloti	548 ± 58	42 ± 1	142 ± 25	392 ± 16	316 ± 238	193 ± 49	2.77 ± 0.50	−3.80-−32.44	0.82-−6.42
P. fluorescens	821 ± 161	150 ± 7	184 ± 22	343 ± 12	152 ± 22	152 ± 122	1.86 ± 0.23	−3.97-−32.33	0.71-−6.25
R. sphaeroides	553 ± 35	86 ± 2	82 ± 20	447 ± 24	33 ± 56	180 ± 25	5.43 ± 1.73	−6.60-−41.34	9.71-−3.62
P. versutus	1048 ± 85	147 ± 19	96 ± 8	421 ± 75	43 ± 13	190 ± 21	4.41 ± 0.87	−8.68-−33.45	1.83-−1.54
B. subtilis	515 ± 117	281 ± 10	154 ± 9	615 ± 44	284 ± 89	245 ± 64	3.98 ± 0.37	−4.71-−29.68	−1.94-−5.51
E. coli	252 ± 46	32 ± 3	204 ± 7	854 ± 59	202 ± 55	320 ± 69	4.18 ± 0.33	−6.40-−30.78	−0.84-−3.82

Data are means ± standard deviations of results from triplicate experiments.

Δ_rG′, estimated range of Gibbs energies of reaction for membrane-bound transhydrogenase (mTH; from NADH toward NADPH with H⁺ transfer) and soluble transhydrogenase (sTH; from NADPH toward NADH, energy independent).