TABLE 3.

Motility phenotypes of A. hydrophila AH-3 in-frame and defined insertion mutants and in trans-complemented mutants

Strain and type of mutant	Gene defect interval or plasmid used	Motility		Flagellation typec
		Swarminga	Swimmingb	Lateral	Polar
In frame and defined insertion
AH-3	Wild type	3.7 ± 0.2	+	+	+
AH-5503	lafK	0.6 ± 0.1	+	−	+
AH-5510	lafTU	0.6 ± 0.1	+	+	+
AH-4444	pomB	3.4 ± 0.1	+	+	+
AH-4448	pomAB	3.5 ± 0.2	+	+	+
AH-4470	pomB2	3.4 ± 0.3	+	+	+
AH-4471	pomA2B22	3.4 ± 0.1	+	+	+
AH-4472	pomB-B2	1.1 ± 0.1	−	+	+
AH-4473	pomAB-A2B22	1.2 ± 0.2	−	+	+
AH-4452	pomAB-B2	1.1 ± 0.1	−	+	+
AH-4449
AH-4450	lafTU-pomAB	0.5 ± 0.1	+	+	+
AH-5511	lafK-pomAB	0.6 ± 0.1	+	−	+
AH-5512	lafK- pomA2B22	0.5 ± 0.1	+	−	+
In trans complemented
AH-4472	pACYC-POMAB	3.3 ± 0.2	+	+	+
	pACYC-POMA2B2	3.4 ± 0.2
AH-4473	pACYC-POMAB	3.2 ± 0.1	+	+	+
	pACYC- POMA2B2	3.4 ± 0.2
AH-4452	pACYC-POMAB	3.2 ± 0.2	+	+	+
	pACYC- POMA2B2	3.1 ± 0.1

^aMigration (in centimeters) of bacteria through the swarm agar plates from the center toward the periphery of the plate.

^b−, no motility in liquid media; +, motility in liquid media.

^c−, absence of flagella; +, flagella equivalent to those of the wild type.