TABLE 3.
CAT activities representing expression of celA in wild-type strain UA159 and manL- and ccpA-deficient mutantsa
| Sugar(s) | PcelA-cat CAT activity (nmol [mg protein]−1 min−1) for indicated strains
|
||
|---|---|---|---|
| manL+ ccpA+ | manL | ccpA | |
| Cellobiose/glucose | 1.78 (0.17) | 795.61 (39.1) | 2.32 (0.13) |
| Cellobiose/fructose | 0.50 (0.32) | 1.15 (0.19) | 0.42 (0.07) |
| Cellobiose/mannose | 0 (0) | 1.27 (0.37) | 1.77 (0.56) |
| Glucose | 0.97 (0.07) | 1,371.95 (92.8) | 1.97 (0.74) |
| Glucose/fructose | NT | 2.04 (0.72) | NT |
| Glucose/mannose | NT | 2.61 (1.72) | NT |
| Glucose/galactose | NT | 1,273.40 (150.90) | NT |
a
Each sugar was added at a concentration of 0.5%, including the mixed sugars. Data represent the average and standard deviation (in parentheses) of three independent assays. NT, not tested.