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Western blot analysis of fractionated S. pneumoniae TIGR4, D39, TIGR4 ply, and D39 ply strains. Ply is found in the cell wall of both wt strains, whereas the cytoplasmic control protein CodY is found exclusively in the protoplast fraction. RrgB, a multimeric cell wall protein specific to TIGR4, is found primarily in the cell wall lane for the two TIGR4 strains. Ply is absent in the ply mutant strain. Sup, culture supernatant; CW, cell wall; Prt, protoplast.
