FIG. 3.

CiaR contributes to GBS resistance to killing by antimicrobial effectors. (A and B) Killing kinetics of WT GBS, the ΔciaR mutant, and the ΔciaR mutant complemented with pciaR upon exposure to 16 μM mCRAMP (A) or 64 μM polymyxin B (B). (C to E) Survival of WT GBS, the ΔciaR mutant, and the complemented strain after exposure to 0.03% H₂O₂ (C), 0.01% hypochlorite (D), or 100 ng/ml of lysozyme (E). The survival index was calculated as total CFU recovered/CFU in the starting inoculum. *, P < 0.05; **, P < 0.01; ***, P < 0.001. All experiments were performed at least three times with similar results. Results of representative experiments are shown.