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Swimming motility assay of Xc17 and Xc17-derived mutant strains. WT, wild-type strain Xc17; flhA(c), flhA mutant complemented with pBBADflhA; flhB(c), flhB mutant complemented with pBBADflhB. For each tube, 3 μl (ca. 105 CFU) mid-log phase culture was inoculated onto the surface of freshly prepared semisolid (0.3% agar) XOL medium and observed after 48 h of incubation.
