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(B) Different amounts of whole-cell extracts were separated on an SDS-polyacrylamide gel. Lane 1, 10 μg Xc17; lane 2, 2 μg flgM mutant; lane 3, 8 μg flgM mutant. (A) This was followed by Western blotting with polyclonal antibodies raised against the flagellin (FliC). (C) Western blotting, using polyclonal antibodies against σ28 (FliA), of the cell extract. Lane 1, Xc17; lane 2, flgM mutant; lane 3, flgM mutant complemented with pRKflgM.
