TABLE 2.
Oligonucleotides used in this work
| Oligonucleotide | Sequence (5′→3′)a | Enzyme site added |
|---|---|---|
| flhAw-F | GGTACCagcgcatgagcgcccaacc | KpnI |
| flhAw-R | AAGCTTccggctcagctaatcgtgcc | HindIII |
| flhBw-F | GGTACCccaatgtccgagtccgaaga | KpnI |
| flhBw-R | AAGCTTgggcgctcatgcgctggccc | HindIII |
| flhB_PstI-F | cgaaggcggcCTGCAGcacc | PstI |
| flhB_PstI-R | ggaactggCTGCAGaagctg | PstI |
| flgM-F | tgttgtcggccaatgacc | |
| flgM-R | Cagtgcccagttcacctc | |
| fliA-F | AGATCTgacgagatccgtcgg | BglII |
| fliA-R | AAGCTTagcgacagcaccagc | HindIII |
| fliAw-F | CATATGatgagtaccgccacc | NdeI |
| fliAw-R | CTCGAGatcgtcaatttcgac | XhoI |
| fliCw-F | CATATGgcacaggtaatcaaca | NdeI |
| fliCw-R | GCGGCCGCctgcagcaggctcagcac | NotI |
| fliCp-F | CTCGAGttgcgcgcaaaaacccgt | XhoI |
| fliCp-R | TCTAGAttttgatatctcctctaa | XbaI |
| flgMw-F | AGATCTGatgagccagaaaatcgaaggc | BglII |
| flgMw-R | GGATCCttgcccgccagttgctggttc | BamHI |
| rpoN2w-F | CATATGaagacgaccatctctg | NdeI |
| rpoN2w-R | CTCGAG tcccgcccgggcaagcag | XhoI |
| fliA_YB-F | CATATGaagatcaaacgctttgttgccc | NdeI |
| fliA_YB-R | GGATCCtcttcaaggcgaagaacgagac | BamHI |
| flgM_YA-F | CATATGagtaccgccaccgcaacgaccg | NdeI |
| flgM_YA-R | GGATCCatcgtcaatttcgacaccggca | BamHI |
a
Uppercase letters indicate the restriction enzyme site.