FIG. 2.

B220⁺ and CD11b⁺ populations in F-DC respond to rwt and rM51R-M viruses. F-DC were infected with rwt or rM51R-M virus at a multiplicity of 1 PFU/cell or treated with Lox or LPS for 24 h. After infection, the expression of costimulatory molecules was measured in combination with the subset markers CD11b or B220 by flow cytometry. The level of expression of CD80 on each gated population is depicted in the histograms shown in panel A. The cell surface expression of CD40 (B), CD80 (C), and CD86 (D) in each sample is shown as the geometric mean fluorescence (left side) and as the increase in costimulatory molecule expression over that in untreated cells (NT) (right side). The data are the means ± the standard errors for three experiments.