FIG. 5.

Mengovirus-induced permeabilization of the nuclear envelope is sensitive to protein kinase inhibitors. Permeabilized uninfected HeLa-3E cells were incubated with the lysates from mengovirus-infected (4 h p.i.) and mock-infected HeLa cells for 1 h at 37°C in the absence or presence of the indicated concentrations of a broad-spectrum protein kinase inhibitor staurosporin and two inhibitors of cycle-dependent kinases, roscovitine and olomoucine. An inactive analog of the latter drug, iso-olomoucine, was used as a control.